Swarm Optimization Techniques for Segmenting Gel Electrophoresis Images
نویسندگان
چکیده
منابع مشابه
An Iterative Algorithm for Segmenting Lanes in Gel Electrophoresis Images
In this paper we discuss the application of spatial-domain filters for solving the problem of automatic lane detection on gel electrophoresis computer images. The problem can be posed as the determination of the number, location and orientation of lanes on the image, based on the analysis of their gray-level intensities. A novel iterative filtering algorithm is proposed based on the periodicity...
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Introduction Two-Dimensional Gel Electrophoresis technique is a convenient and well-established method to separate thousands of proteins on polyacrylamide gels, according to the differences in their net charge and their molecular mass [1]. Its digital output is an image which depicts proteins as bright or dark spots over a noisy and inhomogeneous background. Each protein is characterized by its...
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OBJECTIVES 2D gel electrophoresis (2-DE) is the method of choice for analyzing protein expression in the field of proteomics, for example, comparing a reference with a test population. However, due to complex physical and chemical processes the locations of proteins generally vary in different 2-DE images. To cope with these variations, accurate geometric alignment of 2-DE images is important. ...
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Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues. In this paper, we present a fully automated system with high accuracy for analyzing DNA and proteins. The proposed algorith...
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Despite recent progress in "shotgun" peptide separation by integrated liquid chromatography and mass spectrometry (LC/MS), proteome coverage and reproducibility are still limited with this approach and obtaining enough replicate runs for biomarker discovery is a challenge. For these reasons, recent research demonstrates that there is a continuing need for protein separation by two-dimensional g...
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ژورنال
عنوان ژورنال: American Journal of Bioinformatics
سال: 2016
ISSN: 1948-9862
DOI: 10.3844/ajbsp.2016.18.33